Circadian KaiC Phosphorylation: A Multi-Layer Network
نویسندگان
چکیده
منابع مشابه
Circadian KaiC Phosphorylation: A Multi-Layer Network
Circadian KaiC phosphorylation in cyanobacteria reconstituted in vitro recently initiates a series of studies experimentally and theoretically to explore its mechanism. In this paper, we report a dynamic diversity in hexameric KaiC phosphoforms using a multi-layer reaction network based on the nonequivalence of the dual phosphorylation sites (S431 and T432) in each KaiC subunit. These diverse o...
متن کاملAn allosteric model of circadian KaiC phosphorylation.
In a recent series of ground-breaking experiments, Nakajima et al. [Nakajima M, Imai K, Ito H, Nishiwaki T, Murayama Y, Iwasaki H, Oyama T, Kondo T (2005) Science 308:414-415] showed that the three cyanobacterial clock proteins KaiA, KaiB, and KaiC are sufficient in vitro to generate circadian phosphorylation of KaiC. Here, we present a mathematical model of the Kai system. At its heart is the ...
متن کاملKaiA-stimulated KaiC phosphorylation in circadian timing loops in cyanobacteria.
Cyanobacterial clock proteins KaiA and KaiC are proposed as positive and negative regulators in the autoregulatory circadian kaiBC expression, respectively. Here, we show that activation of kaiBC expression by kaiA requires KaiC, suggesting a positive feedback control in the cyanobacterial clockwork. We found that robust circadian phosphorylation of KaiC. KaiA was essential for in vivo KaiC pho...
متن کاملReconstitution of circadian oscillation of cyanobacterial KaiC phosphorylation in vitro.
Kai proteins globally regulate circadian gene expression of cyanobacteria. The KaiC phosphorylation cycle, which persists even without transcription or translation, is assumed to be a basic timing process of the circadian clock. We have reconstituted the self-sustainable oscillation of KaiC phosphorylation in vitro by incubating KaiC with KaiA, KaiB, and adenosine triphosphate. The period of th...
متن کاملAn allosteric model for circadian KaiC phosphorylation - Supporting Information
3 Full Model of the Kai System 12 3.1 Model: the chemical rate equations . . . . . . . . . . . . . . . . . 12 3.2 Model: The free-energy difference between the active and inactive state of KaiC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 3.3 Setting the parameters . . . . . . . . . . . . . . . . . . . . . . . . 15 3.4 Reduced model . . . . . . . . . . . . . . . . . . . . . . . ...
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ژورنال
عنوان ژورنال: PLoS Computational Biology
سال: 2009
ISSN: 1553-7358
DOI: 10.1371/journal.pcbi.1000568